Journal: The Journal of comparative neurology

Article Title: Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina

doi: 10.1002/cne.24699

Figure Lengend Snippet: Primary antibodies

Article Snippet: Connexin 37 , (C)EHQMAKISVAEDGR , rabbit , 1:2000 , poly , Alomone, ACC-204 , RRID:AB_2722598.

Techniques: Marker, Purification, Recombinant

Journal:

Article Title: Reduction of electrical coupling between microvascular endothelial cells by NO depends on connexin37

doi: 10.1152/ajpheart.01148.2008

Figure Lengend Snippet: Effect of DETA exposure on intercellular resistance in wild-type, connexin (Cx) 37 null, Cx40 null, and Cx43G60S (nonfunctional mutant) MMEC. DETA (500 μM, 3 h) increased resistance in cells derived from wild-type, Cx40 null, and Cx43G60S mice, but not in cells from Cx37 null mice. *Significant difference from the appropriate control group, P < 0.05; n = 16 in group 1 (pooled from all control experiments), n = 21 in group 2 (pooled), and n = 4 in groups 3–8.

Article Snippet: Peroxidase-labeled anti-rabbit IgG antibodies for anti-Cx37, anti-PKG, and anti-phosphoserine antibodies were purchased from Biolynx (Brockville, ON) and Cell Signaling Technology (Beverly, MA).

Techniques: Mutagenesis, Derivative Assay

Journal:

Article Title: Reduction of electrical coupling between microvascular endothelial cells by NO depends on connexin37

doi: 10.1152/ajpheart.01148.2008

Figure Lengend Snippet: Lack of effect of DETA on Cx37, Cx40, and Cx43 protein expression in wild-type MMEC. A: Cx37, Cx40, Cx43, and β-actin immunoblots from control and DETA-treated MMEC (500 μM, 3 h). B: densitometric ratios of Cx37, Cx40, and Cx43 to β-actin immunoblots for control and DETA-treated MMEC. DETA had no effect on Cx37, Cx40, or Cx43 protein expression; n = 3 in each group.

Article Snippet: Peroxidase-labeled anti-rabbit IgG antibodies for anti-Cx37, anti-PKG, and anti-phosphoserine antibodies were purchased from Biolynx (Brockville, ON) and Cell Signaling Technology (Beverly, MA).

Techniques: Expressing, Western Blot

Journal:

Article Title: Reduction of electrical coupling between microvascular endothelial cells by NO depends on connexin37

doi: 10.1152/ajpheart.01148.2008

Figure Lengend Snippet: Lack of effect of DETA on distribution of Cx37, Cx40, and Cx43 within wild-type MMEC. Top: representative examples of control (n = 9) and DETA-treated cells (500 μM, 3 h, n = 6) probed for Cx37. Although most of the immunoreactivity is cytoplasmic, occasional punctate staining (arrows) indicates putative Cx37 gap junctions. Comparable staining was also seen with another anti-Cx37 antibody (i.e., that used for immunoblotting) (data not shown). The superimposed oval uniform gray shapes are cell nuclei identified with Hoechst 33258 stain. Middle: representative examples of control (n = 7) and DETA-treated (n = 7) cells probed for Cx40. Numerous small Cx40 gap junctions are indicated by the punctate staining. The superimposed oval gray shapes are cell nuclei. Bottom: representative examples of control (n = 3) and DETA-treated (n = 3) cells probed for Cx43. Numerous Cx43 gap junctions of various sizes are evident, larger than those containing Cx37 or Cx40, whereas gray ovals indicate cell nuclei. Control experiments where the connexin primary antibodies were omitted showed only gray oval nuclei (data not shown). Bar at bottom right indicates 20-μm scale for all panels.

Article Snippet: Peroxidase-labeled anti-rabbit IgG antibodies for anti-Cx37, anti-PKG, and anti-phosphoserine antibodies were purchased from Biolynx (Brockville, ON) and Cell Signaling Technology (Beverly, MA).

Techniques: Staining, Western Blot